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Annual Review of Pharmacology and Toxicology 45, 51-88

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After blocking, cells were incubated with an anti-BrdU antibody (Thermo Fisher Scientific, MA3-071), followed by incubation with a Fluor 488-conjugated secondary antibody (Abcam, ab150117)

Despite the limitations of our lifespan data, findings from our cross-sectional studies in calorie-restricted mice provide robust evidence that glucagon receptor signaling is required for the healthspan benefits of CR and that glucagon receptor signaling at the liver is required for the CR-induced decrease in hepatic mTOR signaling, a primary driver of aging [17,18,19, 48, 97]

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