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Criteria such as a 20 percent reduction in body weight are often designated as indicators of severe suffering, guiding humane endpoint decisions [43]

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Phenylbutazone, Enoxacin, and Doripenem hydrate were treated at 50, 0.3, and 26 mg/kg (N = 4-6 per group)

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Moreover, transgenic mice overexpressing MECP2 recapitulate numerous behavioral phenotypes observed in R MECP2 KO mice, highlighting the necessity of precise MECP2 level regulation for proper central nervous system function