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2019), namely the Columbia (Col-0) ecotype of Arabidopsis thaliana (L.) as a wild type control and Atgstf8 (At2g47730) and Atgstu19 (At1g78380) T-DNA insertional lines

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Stock solutions of 10 mM ferrous ammonium sulfate and 100 mM ascorbate were prepared in 1 M Tris, pH 8.0