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mansoni scattered over multi clades, most of members concentrated in a single group, suggesting that SmGSTs contained both conserved family members and several members in the process of further diversification
Expression of N-terminal His-tagged human GCLM was induced with 1 mM isopropyl--D-thiogalactoside (IPTG, Wako Pure Chemical Industries, Ltd., Osaka, Japan) at 30C for 5 h in Escherichia coli
It is about the cumulative effect of small, consistent choices
Science 281 , 1309 (1998)
