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Western blot analysis For protein analysis, cells or pieces of liver tissue were washed in PBS and lysed in Lysis Buffer (20 mM Tris-HCl pH8, 1 mM NaCl, 0.5% NP40, 0.5% Triton-X-100, 10% glycerol) supplemented with a protease inhibitor cocktail (SigmaAldrich)

Proceedings of the Royal Society B: Biological Sciences, 284 (1857), 20170356

2.2 Preclinical studies: GABA in diabetes In numerous studies using diverse diabetic rodent models, GABA prevents and/or reverses hyperglycemia

Additionally, stable isotope-labeled internal standards for GSH and Glu were employed to verify the overall reliability of the validation approach

10L of resuspended cells were transferred for input protein and whole cell metabolites were extracted from an additional 10L transferred into 40L of 80% methanol with amino acid standards

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