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The following qRT-PCR conditions were used: 40 cycles of 94 C for 15 s, annealing temperatures as indicated in Table (1) for 30 s, and extension temperature at 72 C for 20 s were performed after 95 C for 4 min

doi: 10.1080/09638288.2017.1383518 583 KoenigJESporAScalfoneNFrickerADStombaughJKnightRet al

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