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Imported cysteine generates free cystine once the protein is broken down and must be exported from the lysosome and reduced in the cytosol to regenerate cysteine

References Coskun, T., Urva, S., Roell, W.C., Qu, H., Loghin, C., Kirchner, T., et al

GHK-Cu helps with the long-term remodeling phase, strengthening the repaired tendon tissue over time

Maiuri et al., 2007)

Cell 188, 21902202.e15

Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark