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Extraction and Quantification of Cuticle Hydrocarbon (CHC) Lipids Mosquitoes were air-dried at room temperature for 48 h and then pooled (2025 female mosquitoes/replicate, 3 replicates for each population/strain), the dry weight of each replicate was measured, and CHC analysis was performed by GCMS and GC-FID as previously described 13,32

Since SP-D is in surfactant, little toxicity and a relatively high immunogenic SP-D tolerance are anticipated in humans

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