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Fourth line defense antioxidants The action of these antioxidants basically involves an adaptation mechanism in which they utilize the signals required for free radicals production and reaction to prevent the formation or reaction of such free radicals

Plasma amino acids Plasma amino acid concentrations were measured by ultra-high performance liquid chromatography (ACQUITY H-Class System, Waters Corporation, MA, USA) using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (Waters AccQ Tag assay kit, MA, USA) and norvaline (Sigma Aldrich, MO, USA) as internal standard as previously described 11

The MRM conditions (parent ion fragment ion

There was no significant difference in the accumulation of metabolite M3 between X19-7 and X19 at most time points and even lower than X19 during some treatment time periods (Figure 2C)

doi: 10.3945/jn.109.104638 170 ukowskiW

Sampson, C