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For the Ni-NTA pull-down assay, C-terminally 3xFLAG-tagged AcvB variants (10 M) and C-terminally His 10 -tagged LpiA (10 M) were incubated with 50 L of NiNTA resin in buffer containing 20 mM Tris-HCl (pH 8.0), 150 mM NaCl, and 0.03% DDM at 4 C for 30 min

This situation combined with the lack of new antibiotics requires effort to identify alternatives that might be used to combat recurrent infections

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When youre done washing contaminating proteins off of your column you can add an elution buffer, and your protein of interest will flow out the bottom of the column (Figure 1)

So, if youre looking to support the thyroid and its T3 and iodine delivery service, KI may be a good choice

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