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Primary sclerosing cholangitis (PSC) and primary biliary cholangitis (PBC) reflect gut-derived immune insults, with microbial metabolites (e.g., TMAO and indoxyl sulfate) exacerbating cholestasis, bile duct injury, and systemic inflammation

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Non-biological agents such as dyes are now often used for this application [126,127], but some biological agents are still used for enzyme separation and isolation, including immobilized inhibitors, substrates, and cofactors [15,10,132134]

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