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Performance assessments were conducted using a cycling ergometer test lasting 13 min, during which respiratory quotient, heart rate, and perceived exertion (Borg scale) were recorded at 1-min intervals

Region-specific quantification of IHC labeling showed that IGF-1 treatment promoted neuronal differentiation, increased subcortical myelination, and attenuated synaptogenesis in a region-dependent and time-dependent manner

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