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4-HNE quantification For quantification of 4-HNE levels in growth plates, extraction was performed according to a previously published protocol 38 Growth plates were dissected and homogenized in extraction buffer (100 mM Tris pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 0.5% sodium deoxycholate, 1 mM phenylmethyl sulfonyl fluoride supplemented with PhosSTOP and cOmplete protease inhibitor cocktail)

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doi: 10.1101/gr.229102 39 BouhaddouMMemonDMeyerBWhiteKMRezeljVVCorrea MarreroMet al

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Polyamines The polyamines are highly cationic molecules that tend to bind nucleic acids with high affinity