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Some protocols suggest treatments over several consecutive days to maximize uptake of the nutrient and receive the best overall benefit
HGF has two individual MET-binding interfaces, which include a high affinity NK1 (N-terminal and first kringle domain) binding site and a low affinity -chain binding site (Matsumoto et al., 2017)
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3 volumes of cold ethanol were added to the resulting supernatant and store at 20 C for 1 h, and then, the samples were centrifuged at 6000 g for 10 min
