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Before the use, we confirmed the liver humanization by the presence of human albumin (11.9 0.8 mg/mL) in the blood of PXB mice which were generated using human hepatocytes derived from a single donor

The remaining amino acids that make up the core of the G-site are more variable across GSTs but are thought to aid in the positioning of GSH in the G-Site [84]

The dose of 100 mol sulforaphane and the 7-day treatment duration were determined empirically from previous studies, including a trial demonstrating induction of antioxidant enzymes in nasal lavage cells of human subjects [59] and improved excretion of air pollutants [56]

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doi: 10.1152/jappl.1993.75.2.566

10.1038/s41598-025-99753-8 129 ZhengL.GuanZ