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Kidneys are an extremely important part of the microbiota-gut-brain axis, the bodys primary filter

Jun;65(6):28795

Blanco) were derived from E14.5 d embryos, SV40 T-antigen transformed, and grown in Dulbeccos modified Eagles medium (DMEM, Corning) containing 10% fetal bovine serum (VWR Life Sciences, Seradigm) and 100 g/mL penicillin (Sigma) at 37 C and 5% CO 2

10.1016/j.pdj.2016.08.001 153

Intracranial injections were performed under deep anaesthesia using vaporized isoflurane (22.5%) and oxygen (2 l min 1 )

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