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Description
Wild type and heterozygous MTHFR gene variants characteristically exhibit hypomethylation [9]
RNAi bacteria growth conditions and treatment RNAi bacteria (HT115) containing the L4440 feeding vector are initially grown on LB tetracycline-ampicillin plates (10 mg/ml tryptone, 5 mg/ml yeast extract, 10 mg/ml NaCl, 15 mg/ml agar, 12.5 g/ml tetracycline, 100 g/ml ampicillin) for 16 h at 37 C
Nanotheranostics 2:168
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Following the incubation period, cell viability was assessed using the CCK-8 assay
doi: 10.3389/FIMMU.2020.00827/FULL 145 MazzoniASalvatiLMaggiLCaponeMVanniASpinicciMet al
