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Significantly, the development of novel matrix systems, such as designed and synthesized specific matrices, nanocomposites matrices, and binary or hybrid matrices, should be incorporated into this optimization and validation process to overcome the inherent limitations of current matrices, such as uneven crystallization, matrix-induced ion suppression, and poor compatibility with tissue types

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Key aspects are highlighted as follows: (I) First-generation organic matrices: This category includes both traditional and novel SOMs, which are suitable for (+)MALDI-MS, (-)MALDI-MS, and (+/-)MALDI-MS analyses

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Knott, S