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, The combination of exoglycosidase digestion with MALDI-TOF MS has been shown to be an effective method for characterizing picomole quantities of glycoconjugates and oligosaccharide structures

Role of corticotropin-releasing factor signaling in stress-related alterations of colonic motility and hyperalgesia

doi: 10.1007/s00726-016-2254-8 70 YangW

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In the absence of a Trx tag to enhance the solubility of the expressed protein, the SRCRD proteins were expressed as inclusion bodies in the precipitate of the E