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Karacabeyli D, Lacaille D

doi: 10.1159/000539005

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The disconnect between short plasma half-life and prolonged therapeutic effects observed in many studies supports tissue-level mechanisms extending beyond circulating peptide concentrations

Experimental studies have explored potential interactions with multiple signaling systems and regulatory pathways, but no single receptor target has achieved broad consensus

Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate