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a prospective cohort study from Saudi Arabia

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The excretory pore is located at midline on the ventral side of the head (IntroFIG 3E)

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The procedure is based on the reduction of Ellmans reagent by SH groups to produce 5,5-dithiobis (2-nitrobenzoic acid) which has an intense yellow color that is measured spectrophoto- metrically at 412 nm using Thermo Scientific Genesis 10S spectropho- tometer
