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Image acquisition and analysis for younger and older vesicles Confocal and STED images were acquired using an Abberior microscope setup (Abberior Instruments GmbH) including Olympus IX83 microscope body and Imspector software (version 16.3.15521-w2209)

This routes limitation is related to the small dose that can be administered, which implies the need to design and formulate a drug with a high loading capacity [16], making the route effective only for very potent drugs [6]