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Most practitioners recommend waiting at least 24-48 hours post-surgery before starting to avoid any potential interference with initial clotting and wound closure

10.1016/j.livres.2019.10.001

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Using a flow cytometry-based assay for intracellular glutathione, we found that although melatonin increased cellular GSH levels, this effect was lost when cells were co-treated with melatonin and IKE ( Figure 6I )

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This Fenton reaction generates hydroxyl radicals (OH) which can abstract protons to initiate lipid peroxidation reactions