biosytech glutathione peroxidase kit Solarbio (GSH-Px/GPX) Activity Assay cell culture media with Glutathione Peroxidase Assay Kit (ab102530) Glutathione peroxidase (GPx) activity assay

In both compartments, melatonin treatment prevented the accumulation of -hydroxybutyrate observed in vehicle-treated cells ( Supplementary Figures 6C, D )

For quantification of branched and unbranched K48/K63 linkages, the peptides were diluted with 50 mM AMBC for neutralization, and incubated in a 20 L reaction with 2 ng/l recombinant glutaminyl-peptide cyclotransferase (QPCT) (R&D Systems) for 6 h at 37 C (Ohtake et al, 2016)

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Collectively, our findings indicate that the LTF e- LTF axis promotes ferroptosis in prostate cancer cells by regulating iron transport and enhancing sensitivity to ferroptosis-inducing agents like RSL3