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The subunit peptides (subunits VIII and X) and catalytic centers (cytochrome b , c 1 , and iron-sulfur protein) were not changed with aging in IFM [16]

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2.5 Gene expression of PBMC 2.5.1 RNA extraction PBMC resuspended in PBS were centrifuged (400 x g, 12 min, 4C, Varifuge 3.0, Heraeus, Hanau, Germany) immediately after density gradient centrifugation