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Determination of gastric juice acidity The gastric juice from each mouse was collected and diluted with 5 ml distilled water and centrifuged (Thermo scientific, Germany) at 4 C for 10 min at 1000 rpm (El-Ashmawy et al

To further investigate whether spermidine regulates autophagy levels in AA, we performed transmission electron microscopy and observed that the number of autophagic vacuoles decreased in AA samples compared to that in healthy donors ( Figure 4 )

Protein disulfide isomerases ensure that native disulfides form and nonnative disulfides are broken between cysteine residues

The work shows that LXA4 acts via the ESR2/LPAR3/Nrf2 axis to provide its anti-inflammatory and anti-ferroptotic actions in vitro cell co-culture and in vivo rat models, offering a novel therapeutic target for the intervention of KOA [110]

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This subsequently inhibits the expression of GPX4, thereby inducing ferroptosis and resulting in kidney injury [134]