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Using bacteriostatic water to mix them allows the mixed solution to remain usable for days or weeks because the preservative prevents bacterial growth

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Cell culture and treatment U251 cells (from ATCC, Manassas, VA, USA) were cultured at 37 C in 5% CO 2 in high glucose DMEM and 10% fetal bovine serum supplemented with penicillin (100 U/ml) and streptomycin (100 g/ml)