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162 ScheinC.NotebornM
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116 SrinivasanV.NetzD
Rabinovitch, M
To test our hypothesis, we deleted genes coding for the periplasmic binding protein OppA or the permease OppC in cells lacking glutathione biosynthesis, creating double mutants gshA oppA and gshA oppC (see Figure 5A for a schematic overview)
Dialyzed ABHD11-Flag was then concentrated, if required, using a centrifugal filter column (Vivaspin 2, 10,000 MWCO) at 700 g and 4 C for 3 min
