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Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator

Madiha S., Batool Z., Shahzad S., et al., Naringenin, a Functional Food Component, Improves Motor and NonMotor Symptoms in Animal Model of Parkinsonism Induced by Rotenone, Plant Foods for Human Nutrition 78, no

In this regard, the superoxide anion scavenging capacity of GSH-CDs was assayed utilizing a SOD enzyme activity kit

Extract lowers advanced glycation end-products and increases live births in older patients with assisted reproductive technology: a randomized controlled trial

Cells were seeded in complete medium consisting of RPMI-1640 supplemented with 10% FCS (Sigma), 1% Penicillin/Streptomycin (GIBCO), 1% L-Glutamine (Sigma), and 55 mM -mercaptoethanol (GIBCO)

Knockouts suffer from lethal paralysis in newborns, which is due to an abolished evoked release of presynaptic vesicles at neuromuscular junctions whereas endplate morphology appears intact 29