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Disentangling the complexity of HGF signaling by combining qualitative and quantitative modeling

Menndez SG, Manucha W

[DOI] [PMC free article] [PubMed] [Google Scholar] 64.Kunz N, Kemper C

Our roGFP2 measurements confirm (i) that dynamic roGFP2 sensing (or the physiological glutathionylation/deglutathionylation of regular surface-exposed cysteinyl residues) requires a glutaredoxin activity, and (ii) that glutathionylated proteins or metabolites should be very transient redox species when GSH and glutaredoxins are present (unless they are stabilized by a kinetic uncoupling mechanism) 5,32,34,35,6164

Cut offs to separate cells with signal from background were determined using change point analysis 83
