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Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates iron homeostasis and sensitivity to ferroptosis independent of IRP1 and FBXL5

The derivatized free amino acids were applied to a 30-cm Pico-Tag free-amino-acid-analysis column (3.9300 mm) equilibrated with buffer A equipped with a Waters HPLC system (510 HPLC pump, 717 automatic sampler, 996 photodiode array detector, and Millennium 32 chromatography manager) and eluted with a linear gradient composed of buffer B (0%, 14%, 20%, 46%, and 100%) at a flow rate of 1 mL/min at 46

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[DOI] [PMC free article] [PubMed] [Google Scholar] 21.Alves-Fernandes D.K., Jasiulionis M.G
