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Bound proteins were eluted by boiling the beads in SDS loading buffer for 5 min at 95 C
This is one area where cutting corners can lead to real consequences

Lipid flipping in the omega-3 fatty-acid transporter
Its principal bioactive constituents, rosavin, salidroside, and tyrosol, are known to modulate HIF-1 stabilization, increase VEGF expression, and enhance cerebral oxygenation in stress models

Lemarie, A., Bourdonnay, E., Morzadec, C., Fardel, O
