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If you used something other than BAC water (like saline), the salt concentration might be causing precipitation

Gler AD, Ecker JL, Lall GS, Haq S, Altimus CM, Liao HW et al (2008) Melanopsin cells are the principal conduits for rodcone input to non-image-forming vision

doi: 10.1016/j.jhazmat.2020.122605 148 LinWTsaiBCDayCHChiuPChenRChenMYet al

Once it freezes, its three-dimensional fold is disrupted and the hormone is inactivated

The results indicated that daily particle administration from 3 dpi to either 5 or 7 dpi reduced the accumulation of monocytes within the lungs and ameliorated clinical signs during infection

They also noted that a higher dose that was still within a fairly typical range seemed to show a greater benefit than a lower dose