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Samples were rinsed twice with acetone water-free, block-stained with 1% uranyl acetate in acetone (stock solution: 20% in MeOH) for 1 h at 4 C, rinsed twice with water-free acetone, and embedded in Epon/Araldite (Merck, Darmstadt, Germany): 66% in acetone overnight, 100% for 1 h at RT and polymerized at 60 C for 20 h

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Melanotan 2 peptide is a synthetic hormone analogue that stimulates melanin production in the skin, leading to a darker complexion resembling a tan