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Cell culture and peptide handling The HCC line HepG2 (American Type Culture Collection (ATCC) Manassas, VA, USA) was maintained in Dulbeccos modified Eagles media (DMEM) with 4.5 g/l glucose (Corning, Corning, NY), 10% fetal bovine serum (Corning), and 100 U/ml penicillin and streptomycin (Gibco)

Nutr Res Rev 22(1):4967 Dos Santos TM, Jnior OVR, Alves VS, Coutinho-Silva R, Savio LEB, Wyse AT (2021) Hyperhomocysteinemia alters cytokine gene expression, cytochrome c oxidase activity and oxidative stress in striatum and cerebellum of rodents