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Following 6 d of puromycin selection (which included 3 d of contact inhibition), approximately 5 million cells per sample were washed with PBS, dissociated using 1 TrypLE Express Enzyme (Gibco, 12604039), washed with PBS again and lysed with 600 l of Pierce IP Lysis Buffer (Thermo Scientific, 87788) containing 1 tablet of cOmplete Mini EDTA-free Protease Inhibitor Cocktail per 10 ml of buffer

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