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To identify specific co-regulated transcripts that modulate the hypoxia response we first considered NMD targets with known downstream phenotypic impacts

polyA RNA was isolated from 500ng of RNA was prepared using NEBNext poly(A) mRNA Isolation at 1/5 reaction, fragmented for 10 minutes, and prepared for short read sequencing using NEBNext Ultra II Directional RNA Library Prep kit for Illumina at 1/10 reaction with 12 cycles of amplification 56

The level of GSH in the experimental group was reduced to half as compared to control group

The newly formed fatty acid chains are continuously elongated by fatty acid synthase, eventually generating products such as palmitic acid (Fig

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