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Prepare working solutions in aqueous buffers immediately before use

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The induction of redox-sensitive pathways during tumor cell proliferation is necessary since cell division presents tremendous energy requirements and the production of metabolites from energy-generating reactions must be buffered to prevent oxidative damage and ultimately cell death [171]

Due to their continual modification of DNAs base composition, ROS constantly threaten the structure, function and stability of the genetic material (Poetsch, 2020) these damages not only impair genome function but may also act as an epigenetic mark to help regulate gene expression (Gruber et al., 2018) The deterioration of pathophysiological conditions can be exacerbated when there is an imbalance in oxidative DNA damage and the resulting mutagenesis processes

Cell-derived components of human milk Human milk is a complex fluid composed of diverse solutes, membrane bound structures and living cells 3,10,11 (Fig

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