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A Roto-rod Series 8 apparatus (IITC Life Sciences, Woodland Hills, CA) was used, which records test results when the animal drops onto the individual sensing platforms below the rotating rod

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Track progress through multiple metrics: scale weight (understanding water fluctuations affect this), body measurements at consistent points, progress photos in consistent lighting and posture, and subjective assessments of how clothing fits

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In our research, mainly four types of interaction like hydrogen bonds, hydrophobic, ionic and water bridges have been observed in TMAO-BSA complex as shown in the plot above in the Fig