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Cells were lyzed in lysis buffer (6 M urea, 0.3 M NaCl, 1 mM EDTA, 10 mM sodium ascorbate, 10 mM sodium azide, 5 mM Trolox, 25 mM Tris/HCl pH 7.5) and equal protein concentrations of triplicate samples were diluted 10-fold in wash buffer (0.5% [v/v] Triton X-100, 0.1% [w/v] SDS, 0.3 M NaCl, 1 mM EDTA, 10 mM sodium ascorbate, 10 mM sodium azide, 5 mM Trolox, 25 mM Tris/HCl pH 7.5) and subjected to streptavidin chromatography

doi: 10.1128/aac.43.12.2915

Some suggest AOD 9604 may preferentially target stubborn fat cells (areas with higher fat storage) and may also exert beneficial effects on cartilage/ bone/ connective tissue (though those are more speculative)

Sowers, A

Thus, future studies should focus on identifying specific and stable metabolic biomarkers and developing multi-targeted therapeutic drugs (Fig

It is important to note here that, in addition to its effects on aldehyde dehydrogenase, disulfiram has many other targets