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Patients with PCOS, which is characterized by a triple pathological network of increased oxidative stress, an unbalanced inflammatory microenvironment, and aberrant vascular remodeling, have dysregulated alterations in uterine molecular markers, which constitute the primary pathological basis for pregnancy failure (Shan et al., 2022)

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Measurement of cytokines in the liver and spleen Liver and spleen tissues homogenized with protein extraction solution (PRO-PREP, iNtRON Biotechnology, Seoul, Korea) and measured the quantity of IL-2, IFN- and TNF- in total proteins (1 mg) using mouse cytokine assay kit (R&D Systems, Minneapolis, MN, USA)

This affects cell differentiation, apoptosis, mobility, and gene expression in stress contexts