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Thermo Fisher Scientific, Waltham, MA USA) and 2,7-dichlorofluorescein diacetate (DCFDA, 2.5 M, Sigma) for 15 min in 0.5 mL of PBS

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The remainder can be transported to the liver via the portal vein circulation, and in there it would be oxidized into TMAO by the host liver flavin monooxygenases (FMO1 and FMO3) (Lang et al., 1998

Shan M, Zhang X, Fang X, Tian J, Song L, Chen Y, et al

A nivel metablico, el hgado destina casi el 80% de la materia prima necesaria para producir creatina, la misma que se usa para generar glutatin (antioxidante clave), melatonina (para dormir bien) y carnitina (para quemar grasa)