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This study attempts to develop and validate a simple HPLC-UV method for the determination of GSH in PC-12 cells

Experimental approachesCC50 calculation of phenformin and atpenin A5 CC 50 calculations were performed using three methods to measure cell viability: the MTT assay, the CellTiter-Glo assay (CTG) from Promega (Cat# G7570), and monitoring cell viability via live cell imaging (IncuCyte)

This phenomenon is reminiscent of autoprotection observed in experimental animals, where initial exposure to the sub-toxic doses of acetaminophen protects rodents from subsequent lethal doses of the drug [59,60]

10.1007/s40279-018-0938-9 Sports Med

Noninvasive in vivo imaging of diabetes-induced renal oxidative stress and response to therapy using hyperpolarized 13C dehydroascorbate magnetic resonance

Clinical trials have corroborated these findings, with KD improving both motor and non-motor symptoms in PD patients [18, 85]