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The RT-PCR mixture contained 5 L 5xQIAGEN OneStep RT-PCR Buffer, 1 L dNTP Mix, 1.5 L of 10 M sense primer, 1.5 L of 10 M antisense primer, 1 L OneStep RT-PCR Enzyme Mix, RNA as described and RNase-free water

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A decrease in the catalytic activity of such enzymes or their complete inactivation can dramatically affect the ability of cells to carry out vital anabolic processes and reduce the adaptive potential of cells