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The most severe form is cerebral or juvenile ALD manifested by inflammatory demyelination of the CNS, adrenocortical atrophy, and a rapidly fatal clinical course resulting from the CNS lesion

[DOI] [PubMed] [Google Scholar] 20.Zalups R.K., Cherian M.G., Barfuss D.W

3) were synthesized by GeneArt (Thermo Fisher) and cloned into a pET21 vector harboring a C-terminal 6x-HIS tag (Novagen) using standard molecular biology techniques

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Isopropyl -d-1-thiogalactopyranoside (IPTG) was added to a final concentration of 1 mM and the culture was further incubated at 18 C overnight