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Cells were then transfected with either the pcDNA3 vector (Invitrogen, Waltham, MA, USA) alone as a negative control or with GSK3 or DYRK1A cDNA using JetOptimus transfection reagent (Polyplus transfection, Illkirch, France) following the manufacturers instructions

University of Minnesota Medical School Research Day, 2011

Lifestyle adjustments: Stress reduction, hydration, and quality sleep all contribute to healing

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Microblading is a semi-permanent eyebrow treatment that involves the use of cosmetic tattooing to create the appearance of full, natural-looking brows with fine, hair-like strokes
