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After washing with PBS, the placental tissues were incubated with secondary antibodies for 1 h at 37C and developed with diaminobenzidine tetrahydrochloride, and the signal was measured using an inverted fluorescence microscope (Olympus, BX53F, Japan)

Overview of common practices in calf raising facilities

CampanatiABianchelliTGesuitaRFotiCMalaraGMicaliGet alComorbidities and treatment patterns in adult patients with atopic dermatitis: Results from a nationwide multicenter study

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The expression of -smooth muscle actin (-SMA), as measured by immunostaining and Western blotting, was used as a marker of PSC activation