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CCK-8 assay HUVECs were evenly seeded into 96-well plates at a density of 1 10^4 cells per well, with a culture medium volume of 100 L per well

The therapist and parent get down on the floor with the child, engage with their interests, and use playful interaction to expand circles of communication, emotional connection, and cognitive complexity
doi: 10.1038/nrn1519 69 MowbrayJ

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