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RNA yield and integrity were initially assessed from absorbance readings at 260 and 280 nm using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, Loughborough, UK), and confirmed using an Agilent Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent Technologies, Wokingham, UK) according to the manufacturers guidelines

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SECOND OPINION SERIES What Causes Undermethylation

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Murray AR, Atkinson L, Mahadi MK, Deuchars SA, Deuchars J (2016) The strange case of the ear and the heart: The auricular vagus nerve and its influence on cardiac control

It combines IGF-1, Sermorelin, and Tesamorelin