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We further detected the protein level of SREBF1 and PLIN2 in GSTM2 knockdown cells treated with PA or GS-4997

For experiments with untagged proteins, samples were treated overnight with 6xHis-TEV protease (Invitrogen) according to the manufacturers instructions

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Phosphorylated type I receptors recruit and phosphorylate Smad1/5/8 receptors, which bind Smad4 to form complexes that translocate to the nucleus to regulate the expression of multiple downstream target genes

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